Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017
Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017
Blog Article
Dengue virus (DENV) is the most prevalent arboviral threat worldwide.This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue).Many sporadic cases and outbreaks have occurred in Senegal since 1970.Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab).Overall, 960 human sera were collected and tested simply southern cat shirt in the field for the presence of viral RNA by real-time RT-PCR.
Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed.Out of 960 suspected cases, 131 were confirmed dengue cases.The majority of confirmed cases were from Louga community.Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%).Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.
1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1).Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period.This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE).Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, argan oil pure purple which allows early cases management.